Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue
نویسندگان
چکیده
منابع مشابه
Mechanical versus enzymatic isolation of stromal vascular fraction cells from adipose tissue
Clinical use of adipose-derived stem cells (ASCs) for a variety of indications is rapidly expanding in medicine. Most commonly, ASCs are isolated at the point of care from lipoaspirate tissue as the stromal vascular fraction (SVF). The cells are immediately administered to the patient as an injection or used to enrich fat grafts. Isolation of ASCs from adipose tissue is a relatively simple proc...
متن کاملUnderstanding Mechanical Emulsification (Nanofat) Versus Enzymatic Isolation of Tissue Stromal Vascular Fraction (tSVF) Cells from Adipose Tissue: Potential Uses in Biocellular Regenerative Medicine
Background: With rapid growth of experiences and techniques in the field of Biocellular Regenerative Medicine, clinical scientists and biotechnical advancements constantly seek to understand and optimize uses of the multipotent heterogeneous cellular populations found in adipose tissue complex. The value of including the living, native bioscaffolding within the adipose tissues has likewise gain...
متن کاملShift toward Mechanical Isolation of Adipose-derived Stromal Vascular Fraction: Review of Upcoming Techniques
Standard isolation of adipose stromal vascular fraction (SVF) requires the use of collagenase and is considered more than "minimally manipulated" by current good manufacturing practice requirements. Alternatively, nonenzymatic isolation methods have surfaced using physical forces to separate cells from the adipose matrix. The purpose of this study was to review the literature on the use of mech...
متن کاملAbstract: Mechanical Isolation of Adipose-Derived Stromal Vascular Fraction: Is It Becoming a Growing Trend?
MaterialS and MethodS: Adults undergoing liposuction on bilateral body areas were included (n=12). Under sedation, liposuction on one side was conducted with standard tumescent (1L of LR with 30ml of 1% lidocaine and 1 mcg/ml epinephrine). Tumescent without lidocaine was infiltrated on the contralateral side. Five milliliter lipoaspirate samples were processed for isolation of the stromal vascu...
متن کاملIsolation of stromal stem cells from human adipose tissue.
The stromal compartment of mesenchymal tissues is thought to harbor stem cells that display extensive proliferative capacity and multilineage potential. Stromal stem cells offer a potentially large therapeutic potential in the field of regenerative medicine. Adipose tissue contains a large number of stromal stem cells, is relatively easy to obtain in large quantities, and thus constitutes a ver...
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ژورنال
عنوان ژورنال: SpringerPlus
سال: 2015
ISSN: 2193-1801
DOI: 10.1186/s40064-015-1509-2